Neuron number in the myenteric plexus of the ascending colon of rats: a comparative study using two staining techniques

Arquivos de Neuro-Psiquiatria

Débora M. G. Sant'ana²  Marcílio H. Miranda-neto²  Sonia L. Molinari²  Marco Antônio Sant'anna¹ 

[1] ,Universidade Estadual de Maringá

关键词: myenteric plexus;    ascending colon;    enteric neurons;    plexo mientérico;    colo ascendente;    neurônios entéricos;   

DOI  :  10.1590/S0004-282X1997000300018

来源: SciELO

【 摘 要 】

We carried out this study with the purpose of comparing the neuronal density in antimesocolic and intermediate regions of the colon of rats. We used the ascending colon of ten seven-months old Wistar rats. With the Giemsa method we found 29046 neurons/cm² on the antimesocolic region and 30968 neurons/cm² on the intermediate regions. With the NADH-diaphorase technique 12308 neurons/cm² on the antimesocolic regions and 8798 neurons/cm² on the intermediate regions were evidenced. The number of NADH-diaphorase positive neurons is significantly less than the number of Giemsa-stained neurons, and that this difference is enhanced on the intermediate regions of the intestinal circumference. Therefore, to compare the number of neurons of an intestinal segment of a same species at the same age, it is necessary to take into consideration the technique employed and the region of the intestinal circumference from where the sample was obtained.

【 授权许可】

CC BY   
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