Protective effect of N2-mercaptopropionylglycine on rats and dogs liver during ischemia/reperfusion process

Arquivos de Gastroenterologia

Emilio Elias Abdo¹  José Eduardo Monteiro Cunha¹  Pérsio Deluca¹  Ana Maria Mendonça Coelho¹  Telesforo Bacchella¹  Marcel Cerqueira César Machado¹ 

[1] ,University Medical School Department of Gastroenterology Surgical DivisionSão Paulo SP ,Brazil

关键词: Mercaptopropionylglicyne;    Ischemia;    Reperfusion;    Liver diseases;    Rats;    Dogs;    Mercaptopropionilglicina;    Isquemia;    Reperfusão;    Hepatopatias;    Ratos;    Cães;   

DOI  :  10.1590/S0004-28032003000300008

来源: SciELO

【 摘 要 】

BACKGROUND: N2-mercaptopropionylglycine is a powerful super oxide synthesis inhibitor and has been tested as a preventive agent of metabolic and structural hepatic damage in the ischemia/reperfusion process. AIM: To analyze some effects of N2-mercaptopropionylglycine administration to animals of two species submitted to normothermic liver ischemia/reperfusion. MATERIAL AND METHODS: Twenty-two rats and 22 dogs were divided into four groups: group I: rats that received intravenous saline 0.9%; group II: rats that received 100 mg/kg of N2-mercaptopropionylglycine; group III: dogs that received saline intravenous 0.9% and group IV: dogs that received 100 mg/kg N2-mercaptopropionylglycine. RESULTS: Ten minutes after the saline or drug administration, each group was submitted to left lobe liver ischemia for 25 minutes followed by reperfusion. Biochemical studies 24 hours after reperfusion revealed a significantly lower elevation of transaminases in animals of groups II (AST = 271 ± 182; ALT = 261 ± 161 ) and IV (AST = 101 ± 45; ALT = 123 ± 89) when compared to the controls group: I (AST = 2144 ± 966; ALT = 1869 ± 1040 00) and III (AST = 182 ± 76.51; ALT = 277 ± 219), respectively. Histology study demonstrated a significantly minor aggression to animals of groups II and IV when compared to groups I and III, respectively. CONCLUSION: These results suggest a significant release of free radicals of oxygen in the process and that N2-mercaptopropionylglycine may have a significant protective effect on liver parenchyma when submitted to ischemia/reperfusion.

【 授权许可】

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