Subconjunctival and topical application of recombinant tissue plasminogen activator in rabbits

Arquivos Brasileiros de Oftalmologia

José Ricardo De Abreu Reggi¹  Richard Yudi Hida¹  Milton Massato Hida¹  Maria Cristina Nishiwaki-dantas¹  Hisashi Suzuki¹ 

关键词: Tissue plasminogen activator;    Anterior chamber;    Hyphema;    Inflammation;    Postoperative complications;    Animals;    Rabbits;    Ativador de plasminogênio tecidual;    Câmera anterior;    Hifema;    Complicações pós-operatórias;    Animais;    Coelhos;   

DOI  :  10.5935/0004-2749.20150003

来源: SciELO

【 摘 要 】

Purpose: To quantify fibrin degradation products after topical and subconjunctival administration of recombinant tissue plasminogen activator in rabbits. Methods: Fibrin formation was induced in the anterior chamber in 25 rabbits. Subsequently, five rabbits received an injection of r-TPA (positive control) in the anterior chamber, another 10 received a subconjunctival injection of r-TPA, and the remaining 10 received instillations of topical r-TPA. Afterwards, samples of aqueous humor were collected and semi-quantitative analysis of fibrin degradation products (FDP) was performed. Results: No statistical differences were noted between the treatment and control groups at any time point. Fibrin degradation products semi-quantification showed statistical improvement in the control group and the subconjunctival group. Conclusion: Fibrin degradation products were observed in the anterior chamber after subconjunctival administration of r-TPA. However, it was probably not sufficient to cause fibrin degradation. Topical r-TPA did not effectively absorb anterior chamber fibrin.

【 授权许可】

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