Antimicrobial susceptibility of photodynamic therapy (UVA/riboflavin) against Staphylococcus aureus

Arquivos Brasileiros de Oftalmologia

Renata Tiemi Kashiwabuchi²  Yasin Khan¹  Fabio Ramos De Souza Carvalho²  Flavio Hirai²  Mauro Silveira Campos²  Peter John Mcdonnell¹ 

[1] ,Universidade Federal de São Paulo - UNIFESP Escola Paulista de Medicina Department of OphthalmologySão Paulo,Brazil

关键词: Keratitis;    Riboflavin;    Corneal ulcer;    Staphylococcus aureus;    Ultraviolet rays;    Cross-linking reagents;    Ceratite;    Riboflavin;    Úlcera da córnea;    Staphylococcus aureus;    Raios ultravioleta;    Reagentes para ligações cruzadas;   

DOI  :  10.1590/S0004-27492012000600011

来源: SciELO

【 摘 要 】

PURPOSE: To assess S. aureus in vitro viability after the exposure to ultraviolet light A (UVA) and riboflavin (B2). METHODS: Samples of S. aureus in 96 well plates (in triplicate) were exposed to riboflavin (B2) and ultraviolet light A (365 nm wavelength) at a power density of 3 mW/cm², 8 mm spot diameter, for 30 minutes. Control groups were prepared as well in triplicate: blank control, ultraviolet light A only, riboflavin only and dead bacteria Control. The bacterial viability was measured using fluorescent microscopy. In order to investigate the occurrence of "viable but non-culturable" microorganisms after treatment, the cell viability was also investigated by plate culture procedure onto a broth medium. Statistical analysis was performed using the triplicate values from each experimental condition. RESULTS: No difference was observed among the treatment group and the control samples (p=1). CONCLUSION: The combination of riboflavin 0.1% and ultraviolet light A at 365 nm did not exhibit antimicrobial activity against oxacillin susceptible S. aureus.

【 授权许可】

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