Arquivos Brasileiros de Oftalmologia | |
Antimicrobial susceptibility of photodynamic therapy (UVA/riboflavin) against Staphylococcus aureus | |
Renata Tiemi Kashiwabuchi2  Yasin Khan1  Fabio Ramos De Souza Carvalho2  Flavio Hirai2  Mauro Silveira Campos2  Peter John Mcdonnell1  | |
[1] ,Universidade Federal de São Paulo - UNIFESP Escola Paulista de Medicina Department of OphthalmologySão Paulo,Brazil | |
关键词: Keratitis; Riboflavin; Corneal ulcer; Staphylococcus aureus; Ultraviolet rays; Cross-linking reagents; Ceratite; Riboflavin; Úlcera da córnea; Staphylococcus aureus; Raios ultravioleta; Reagentes para ligações cruzadas; | |
DOI : 10.1590/S0004-27492012000600011 | |
来源: SciELO | |
【 摘 要 】
PURPOSE: To assess S. aureus in vitro viability after the exposure to ultraviolet light A (UVA) and riboflavin (B2). METHODS: Samples of S. aureus in 96 well plates (in triplicate) were exposed to riboflavin (B2) and ultraviolet light A (365 nm wavelength) at a power density of 3 mW/cm², 8 mm spot diameter, for 30 minutes. Control groups were prepared as well in triplicate: blank control, ultraviolet light A only, riboflavin only and dead bacteria Control. The bacterial viability was measured using fluorescent microscopy. In order to investigate the occurrence of "viable but non-culturable" microorganisms after treatment, the cell viability was also investigated by plate culture procedure onto a broth medium. Statistical analysis was performed using the triplicate values from each experimental condition. RESULTS: No difference was observed among the treatment group and the control samples (p=1). CONCLUSION: The combination of riboflavin 0.1% and ultraviolet light A at 365 nm did not exhibit antimicrobial activity against oxacillin susceptible S. aureus.
【 授权许可】
CC BY
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