Use of a gel biopolymer for the treatment of eviscerated eyes: experimental model in rabbits

Arquivos Brasileiros de Oftalmologia

Francisco De Assis Cordeiro-barbosa¹  José Lamartine De Andrade Aguiar¹  Mariana Montenegro De Melo Lira¹  Nicodemos Teles De Pontes Filho¹  Sidcley Bernardino-araújo¹ 

[1] ,Universidade Federal de Pernambuco Department of Surgery Laboratory of Immunopathology KeizoAssamiRecife PE ,Brazil

关键词: Eye enucleation;    Eye evisceration;    Bioprosthesis;    Eye;    artificial;    Saccharum;    Orbital implants;    Biopolymers;    Models;    animal;    Rabbits;    Enucleação ocular;    Evisceração do olho;    Bioprótese;    Olho artificial;    Saccharum;    Implantes orbitários;    Biopolímeros;    Modelos animais;    Coelhos;   

DOI  :  10.1590/S0004-27492012000400010

来源: SciELO

【 摘 要 】

PURPOSE: To evaluate histologically the integration process of cellulose gel produced by Zoogloea sp when implanted into rabbits' eviscerated eyes. METHODS: This experimental study employed 36 eyes of 18 rabbits subjected to evisceration of their right eyes. The sclerocorneal bag was sutured and filled with biopolymer from sugar cane in the gel state. All animals were clinically examined by biomicroscopy until the day of their sacrifice which occurred on the 7th, 30th, 60th, 90th, 120th, or 240th day. The eyeballs obtained, including the left eyes considered controls were sent for histopathological study by optical macroscopy and microscopy. Tissue staining techniques used included hematoxylin-eosin, Masson trichrome (with aniline), Gomori trichrome, Van Gienson, Picrosirius red, and periodic acid-Schiff (PAS). RESULTS: No clinical signs of infection, allergy, toxicity, or extrusion were observed throughout the experiment. The corneas were relatively preserved. Macroscopic examination revealed a decrease of ~ 8% in the volume of the bulbs implanted with the biopolymer. After cutting, the sclerocorneal bag was solid, compact, elastic, and resistant to traction, with a smooth and whitish surface, and showed no signs of necrosis or liquefaction. The episcleral tissues were somewhat hypertrophied. The histological preparations studied in different colors revealed an initial lymphoplasmacytic infiltration, replaced by a fibroblastic response and proliferation of histiocytes, along with formation of giant cells. Few polymorphonuclearneutrophils and eosinophils were also found. Neovascularization and collagen deposition were present in all animals starting from day 30; although on the 240th day of the experiment the chronic inflammatory response, neovascularization and collagen deposition had not yet reached the center of the implant. CONCLUSION: In this model, the cellulose gel produced by Zoogloea sp proved to be biocompatible and integrated into the orbits. Morphometric, immunohistochemical and biodegradability studies should be performed in the future.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License