Testing intravitreal toxicity of rapamycin in rabbit eyes

Arquivos Brasileiros de Oftalmologia

Roberta Pereira De Almeida Manzano²  Gholam A. Peyman¹  Palwasha Khan¹  Muhamet Kivilcim¹  Patricia Chevez-barrios¹  Walter Takahashi² 

[1] ,Universidade de São Paulo Department of Ophthalmology São Paulo SP ,Brazil

关键词: Choroidal neovascularization;    Sirolimus;    Uveitis;    Vascular endothelial growth factor receptor-1;    Rabbits;    Neovascularização coroidal;    Sirolimo;    Uveite;    Receptor 1 do fator de crescimento do endotélio vascular;    Coelhos;   

DOI  :  10.1590/S0004-27492009000100004

来源: SciELO

【 摘 要 】

PURPOSE: To evaluate retinal toxicity of varying doses of rapamycin when injected intravitreally in rabbits. Rapamycin is a potent immunosuppressive agent with significant antitumor and antiangiogenic properties, clinically approved for prevention of organ transplant rejection. METHODS: Twelve New Zealand albino rabbits were divided into four groups. Four different doses of rapamycin were prepared in 0.1 ml: 20 µg, 50 µg, 200 µg, and 1000 µg. Each concentration was injected in one eye of three rabbits, and 0.1 ml volume of sterile BSS was injected into the contralateral eye of the three rabbits. Slit-lamp and fundoscopic examinations were performed and the animals were observed for 2 weeks for signs of infection, inflammation, and toxicity. A baseline ERG was performed before drug treatment and at day 14, after which the rabbits were euthanized. Histology of the enucleated eyes was studied to look for retinal toxicity. RESULTS: ERG results showed some decrease in scotopic response; however this was not dose related. ERG results were normal at 20 µg. Histological results showed no retinal toxicity in all groups. CONCLUSION: Although ERG changes were identified at dosages between 50-1000 µg, the histology of all groups up to 1000 µg did not show any discernable abnormalities.

【 授权许可】

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