Counteracting effects on free radicals and histological alterations induced by a fraction with casearins

Anais da Academia Brasileira de Ciências

Éverton JosÉ Ferreira De AraÚjo¹  Guilherme AntÔnio Lopes De Oliveira¹  LÍvia Queiroz De Sousa¹  Vanderlan Da Silva Bolzani¹  Alberto JosÉ Cavalheiro¹  Adriana Da Rocha Tome¹  Ana Paula Peron¹  AndrÉ Gonzaga Dos Santos¹  Antonia Maria Das GraÇas Lopes CitÓ¹  ClÁudia Pessoa¹  Rivelilson Mendes De Freitas¹  Paulo Michel Pinheiro Ferreira¹ 

关键词: Clerodane diterpenes;    Casearia sylvestris;    antioxidant;    morphological changes;    toxicity;    Diterpenos clerodânicos;    Casearia sylvetris;    antioxidante;    alterações morfológicas;    toxicidade;   

DOI  :  10.1590/0001-3765201520150149

来源: SciELO

【 摘 要 】

ABSTRACTCasearia sylvestris Swartz is a medicinal plant widely distributed in Brazil. It has anti-inflammatory, antiulcer and antitumor activities and is popularly used to treat snakebites, wounds, diarrhea, flu and chest colds. Its leaves are rich in oxygenated tricyclic cis-clerodane diterpenes, particulary casearins. Herein, we evaluated the antioxidant activities of a fraction with casearins (FC) isolated from C. sylvestrisand histological changes on the central nervous system and livers of Mus musculus mice. Firstly, in vitro studies (0.9, 1.8, 3.6, 5.4 and 7.2 μg/mL) revealed EC50 values of 3.7, 6.4 and 0.16 µg/mL for nitrite, hydroxyl radical and TBARS levels, respectively. Secondly, FC (2.5, 5, 10 and 25 mg/kg/day) was intraperitoneally administered to Swiss mice for 7 consecutive days. Nitrite levels in the hippocampus (26.2, 27.3, 30.2 and 26.6 µM) and striatum (26.3, 25.4, 34.3 and 27.5 µM) increased in all treated animals (P < 0.05). Lower doses dropped reduced glutathione, catalase and TBARS levels in the hippocampus and striatum. With the exception of this reduction in TBARS formation, FC displayed only in vitro antioxidant activity. Animals exhibited histological alterations suggestive of neurotoxicity and hepatotoxicity, indicating the need for precaution regarding the consumption of medicinal formulations based on Casearia sylvestris.

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