Epigallocatechin-3-gallate combined with alpha lipoic acid attenuates high glucose-induced receptor for advanced glycation end products (RAGE) expression in human embryonic kidney cells

Anais da Academia Brasileira de Ciências

Jyh-gang Leu¹  Chin-yao Lin¹  Jhin-hao Jian¹  Chin-yu Shih¹  Yao-jen Liang¹ 

关键词: diabetic nephropathy;    receptor of advanced glycation end products;    epigallocatechin gallate;    alpha lipoic acid;    anti-oxidant;    nefropatia diabetica;    receptores de produtos finais da glicacao avancada;    epigalocatequina galato;    acido alfa-lipoico;    antioxidante;   

DOI  :  10.1590/S0001-37652013005000023

来源: SciELO

【 摘 要 】

The anti-oxidant effects of epigallocatechin gallate (EGCG) and alpha lipoic acid (ALA) have been demonstrated in previous studies. The kidney protection effects of EGCG and ALA in patients with kidney injury are still under investigation. The purpose of this study is to investigate the anti-inflammatory and anti-oxidant effects of EGCG and ALA on high glucose-induced human kidney cell damage. EGCG inhibited high glucose(HG)-induced TNF-α and IL-6 production in human embryonic kidney (HEK) cells. Both EGCG and ALA decreased HG-induced receptor of advanced glycation end products (RAGE) mRNA and protein expressions in HEK cells. EGCG and ALA also recovered HG-inhibited superoxide dismutase production and decreased ROS expressions in HEK cells. The synergism of EGCG and ALA was also studied. The effect of EGCG combined with ALA is greater than the effect of EGCG alone in all anti-inflammation and anti-oxidant experiments. Our studies provide a potential therapeutic application of EGCG and ALA in preventing progression of diabetic nephropathy.

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