Caspase-3 activation and increased procollagen type I in irradiated hearts

Anais da Academia Brasileira de Ciências

Samara C. Ferreira-machado¹  Camila Salata¹  Nazareth N. Rocha¹  Alexandre F.s. Corrêa¹  Suzana Côrte-real¹  Antônio A.f. Peregrino¹  Vera M.a. De Campos¹  Cherley B.v. Andrade¹  Mario Bernardo-filho¹  Januário B. Cabral-neto¹  Carlos E. Dealmeida¹ 

关键词: apoptosis;    caspase-3;    fibrosis;    heart;    ionizing radiation;    TGF-β1;    apoptose;    caspase-3;    fibrose;    coração;    radiação ionizante;    TGF-β1;   

DOI  :  10.1590/S0001-37652013005000009

来源: SciELO

【 摘 要 】

The caspase-3-cleaved presence was evaluated in this study in the heart of irradiated rats, during the decline of ventricular function. Female Wistar rats were irradiated with a single dose of radiation (15 Gy) delivered directly to the heart and the molecular, histological and physiological evaluations were performed at thirteen months post-irradiation. The expressions of procollagen type I, TGF-ß1 and caspase-3-cleaved were analyzed using Western blotting. Cardiac structural and functional alterations were investigated by echocardiography and electron microscopy. In the irradiated group, the levels of procollagen type I, TGF-ß1 and caspase-3-cleaved are increased. Significant histological changes (degeneration of heart tissue and collagen deposition) and functional (reduced ejection fraction) were observed. Data suggest that the cardiac function decline after exposure to ionizing radiation is related, in part, to increased collagen and increased caspase-3-cleaved.

【 授权许可】

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