Production, characterization and application of inulinase from fungal endophyte CCMB 328

Anais da Academia Brasileira de Ciências

Diego S. Nascimento¹  Gildomar Valasques Junior¹  Pedro Fernandes²  Geise C.a. Ribeiro¹  Danyo M. Lima¹  Aristóteles Góes-neto²  Rodrigo Q. Oliveira¹  Rita De Cassia L. Figueiredo-ribeiro²  Sandra A. De Assis¹ 

[1] ,Universidade Estadual de Feira de Santana Departamento de Saúde Laboratório de Enzimologia e Tecnologia de FermentaçãoFeira de Santana BA ,Brasil

关键词: Inulin;    inulinase;    yeasts;    semi-arid;    fungal endophyte;    Inulina;    inulinase;    leveduras;    semi-árido;    fungo endofítico;   

DOI  :  10.1590/S0001-37652012005000035

来源: SciELO

【 摘 要 】

Inulinase (β-2,1-D- fructan fructanohydrolase), EC 3.2.1.7, targets the β-2,1 linkage of inulin, a polyfructan consisting of linear β-2,1 linked fructose, and hydrolyzes it into fructose. This use provides an alternative to produce fructose syrup through the hydrolysis of inulin. The objective of this work was to study the production, characterization and applications of inulinases from the fungal endophyte CCMB 328 isolated from the Brazilian semi-arid region. Response Surface Methodology (RSM) was employed to evaluate the effect of variables (concentration of glucose and yeast extract), on secreted inulinase activities detected in the culture medium and also in the inulin hydrolysis. The results showed that the best conditions for inulinase production by CCMB 328 are 9.89 g / L for glucose and 1.09 g / L for yeast extract. The concentration of 0.20 mol/L of NaCl and KCl increased the activity of inulinase from CCMB 328 by approximately 63% and 37%, respectively. The results also showed that the inulinase has potential for inulin hydrolysis, whose conversion yields roughly 72.48 % for an initial concentration of inulin at 1% (w/v).

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