Larvicidal activity of the water extract of Moringa oleifera seeds against Aedes aegypti and its toxicity upon laboratory animals

Anais da Academia Brasileira de Ciências

Paulo M.p. Ferreira²  Ana F.u. Carvalho²  Davi F. Farias²  Nara G. Cariolano²  Vânia M.m. Melo²  Maria G.r. Queiroz¹  Alice M.c. Martins¹  Joaquim G. Machado-neto¹ 

[1] ,Universidade Federal do Ceará Departamento de Biologia Fortaleza CE ,Brasil

关键词: Aedes aegypti;    Daphnia magna;    larvicidal activity;    Moringa oleifera seeds;    toxicity;    Aedes aegypti;    Daphnia magna;    atividade larvicida;    sementes de Moringa oleifera;    toxicidade;   

DOI  :  10.1590/S0001-37652009000200007

来源: SciELO

【 摘 要 】

In this work, biological effects of the water extract of Moringa oleifera seeds (WEMOS) were assessed on eggs and 3rd instar larvae of Aedes aegypti and on its toxicity upon laboratory animals (Daphnia magna, mice and rats). Crude WEMOS showed a LC50 value of 1260µg/mL, causing 99.2 ± 2.9% larvae mortality within 24 h at 5200µg/mL, though this larvicidal activity has been lost completely at 80ºC/10 min. WEMOS did not demonstrate capacity to prevent egg hatching. After extensive dialyses of the crude WEMOS into watersoluble dialyzable (DF) and nondyalizable (NDF) fractions, only DF maintained its efficacy to kill larvae. Acute toxicity evaluations on daphnids (EC50 of 188.7µg/mL) and mice (LD50 of 446.5 mg/kg body weight) pointed out to low toxicity. Despite the thymus hypertrophy, WEMOS revealed to be harmless in orally and subacutelytreated rats. In conclusion, WEMOS has thermostable bioactive compounds against Ae. aegypti larvae with apparent molecular mass lower than 12 kDa and moderately toxic potential.

【 授权许可】

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