Stereological tools in biomedical research

Anais da Academia Brasileira de Ciências

Carlos A. Mandarim-de-lacerda¹ 

[1] ,Universidade do Estado do Rio de Janeiro Instituto de Biologia Departamento de AnatomiaRio de Janeiro,Brasil

关键词: morphometry;    stereology;    volume density;    surface density;    length density;    disector;    fractionator;    morfometria;    estereologia;    densidade de volume;    densidade de superfície;    densidade de comprimento;    disector;    fractionator;   

DOI  :  10.1590/S0001-37652003000400006

来源: SciELO

【 摘 要 】

Stereological studies are more and more frequent in literature, particularly in the development/evolution, pathology, and neurosciences areas. The stereology challenge is to understand the structural inner threedimensional arrangement based on the analysis of the structure slices only showing two-dimensional information. Cavalieri and Scherle's methods to estimate volume, and Buffon's needle problem, are commented in the stereological context. A group of actions is needed to appropriately quantify morphological structures (unbiased and reproducibly), e.g. sampling, isotropic and uniform randomly sections (Delesse's principle), and updated stereological tools (disector, fractionator, nucleator, etc). Through the correct stereology use, a quantitative study with little effort could be performed: efficiency in stereology means a minimum slices sample counting (little work), low cost (slices preparation), but good accuracy. In the present text, a short review of the main stereological tools is done as a background basis to non-expert scientists.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License