Genetic modification of mammalian genome at chromosome level

Anais da Academia Brasileira de Ciências

Oleg L. Serov²  Natalia M. Matveeva¹  Irina A. Serova²  Pavel M. Borodin² 

[1] ,Federal University of Rio de Janeiro Institute of Biophysics Rio de Janeiro,Brazil

关键词: chromosome biotechnology;    Cre/loxP;    ES cells;    MAC;   

DOI  :  10.1590/S0001-37652000000300012

来源: SciELO

【 摘 要 】

The review is concerned with a progress in genetic modification of a mammalian genome in vitro and in vivo at chromosomal level. Recently three new approaches for the chromosome biotechnology have been developed: Using Cre/loxP-system a researcher is able to produce targeted rearrangements of whole chromosomes or their segments or particular genes within the genome, and therefore to modify the set, position and copy number of the endogenous elements of the genome. Mammalian artificial chromosomes (MACs) provide a possibility to introduce into genome relatively large segments of alien chromosome material, either artificially constructed or derived from the genome of different species. Using ES-somatic cell hybrids allows to transfer whole chromosomes or their fragments between different genomes within and between species. Advantages and limitations of these approaches are discussed.

【 授权许可】

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