Electrochemical generation of microbubbles by carbon nanotube interdigital electrodes to increase the permeability and material uptakes of cancer cells

Drug Delivery

Fereshteh Abbasvandi¹  Amirali Ebadi²  Shahriar Shalileh³  Pouya Soltan Khamsi³  Mohammad Salemizadeh Parizi³  Mohammad Abdolahad³  Mohammad Saghafi³  Saeid Ansaryan³  Shohreh Vanaei⁴  Mohammad Ali Khayamian⁵ 

[1] ATMP Department, Breast Cancer Research Center, Motamed Cancer Institute, ACECR, Tehran, Iran;MEMS and NEMS Laboratory, Department of Electrical and Computer Engineering, Faculty of Engineering, University of Tehran, Tehran, Ira;Nano Electronic Center of Excellence, Nano Bio Electronic Devices Lab, School of Electrical and Computer Engineering, University of Tehran, Tehran, Iran;Nano Electronic Center of Excellence, Thin Film and Nanoelectronic Lab, School of Electrical and Computer Engineering, University of Tehran, Tehran, Iran;Nano Electronic Center of Excellence, Nano Bio Electronic Devices Lab, School of Electrical and Computer Engineering, University of Tehran, Tehran, Iran;Nano Electronic Center of Excellence, Thin Film and Nanoelectronic Lab, School of Electrical and Computer Engineering, University of Tehran, Tehran, Iran;School of Biology, College of Science, University of Tehran, Tehran, Iran;Nano Electronic Center of Excellence, Nano Bio Electronic Devices Lab, School of Electrical and Computer Engineering, University of Tehran, Tehran, Iran;Nano Electronic Center of Excellence, Thin Film and Nanoelectronic Lab, School of Electrical and Computer Engineering, University of Tehran, Tehran, Iran;School of Mechanical Engineering, College of Engineering, University of Tehran, Tehran, Iran;

关键词: Microbubble generation;    electrochemistry;    sonoporation;    ultrasonic stimulation;    drug delivery;   

DOI  :  10.1080/10717544.2019.1662514

来源: publisher

【 摘 要 】

Artificial cavitation as a prerequisite of sonoporation, plays an important role on the ultrasound (US) assisted drug delivery systems. In this study, we have proposed a new method of microbubble (MB) generation by local electrolysis of the medium. An integrated interdigital array of three-electrode system was designed and patterned on a nickel-coated quartz substrate and then, a short DC electrical pulse was applied that consequently resulted in distributed generation of microbubbles at the periphery of the electrodes. Growth of the carbon nanotube (CNT) nanostructures on the surface of the electrodes approximately increased the number of generated microbubbles up to 7-fold and decreased their average size from ∼20 µm for bare to ∼7 µm for CNT electrodes. After optimizing the three-electrode system, biocompatibility assays of the CNT electrodes stimulated by DC electrical micropulses were conducted. Finally, the effect of the proposed method on the sonoporation efficiency and drug uptake of breast cells were assessed using cell cycle and Annexin V/PI flow cytometry analysis. These results show the potential of electrochemical generation of MBs by CNT electrodes as an easy, available and promising technique for artificial cavitation and ultrasound assisted drug delivery.

【 授权许可】

CC BY