A journey from the endothelium to the tumor tissue: distinct behavior between PEO-PCL micelles and polymersomes nanocarriers

Drug Delivery

Laure Gibot¹  Muriel Golzio¹  Marie-Pierre Rols¹  Agathe Figarol²  Barbara Lonetti³  Anne-Françoise Mingotaud³ 

[1] Institut de Pharmacologie et Biologie Structurale, IPBS, Université de Toulouse, CNRS, UPS, Toulouse, France;Institut de Pharmacologie et Biologie Structurale, IPBS, Université de Toulouse, CNRS, UPS, Toulouse, France;Laboratoire des IMRCP, Université de Toulouse CNRS UMR, Toulouse, Franc;Laboratoire des IMRCP, Université de Toulouse CNRS UMR, Toulouse, Franc;

关键词: Nanocarriers;    polymers;    self-assembly;    drug delivery;    translocation;    endothelium;    tumor;   

DOI  :  10.1080/10717544.2018.1510064

来源: publisher

【 摘 要 】

Polymeric nanocarriers must overcome several biological barriers to reach the vicinity of solid tumors and deliver their encapsulated drug. This study assessed the in vitro and in vivo passage through the blood vessel wall to tumors of two well-characterized polymeric nanocarriers: poly(ethyleneglycol-b-ε-caprolactone) micelles and polymersomes charged with a fluorescent membrane dye (DiO: 3,3'-dioctadecyloxacarbo-cyanine perchlorate). The internalization and translocation from endothelial (human primary endothelial cells HUVEC) to cancer cells (human tumor cell line HCT-116) was studied in conventional 2D monolayers, 3D tumor spheroids, or in an endothelium model based on transwell assay. Micelles induced a faster DiO internalization compared to polymersomes but the latter crossed the endothelial monolayer more easily. Both translocation rates were enhanced by the addition of a pro-inflammatory factor or in the presence of tumor cells. These results were confirmed by early in vivo experiments. Overall, this study pointed out the room for the improvement of polymeric nanocarriers design to avoid drug losses when crossing the blood vessel walls.

【 授权许可】

CC BY