A multiplex PCR assay for the identification of five species of the Anopheles barbirostris complex in Thailand

Parasites & Vectors

Atiporn Saeung¹  Chutipong Sukkanon²  Theeraphap Chareonviriyaphap²  Michael J. Bangs³  Chanya Udom⁴  Sylvie Manguin⁵  Laura Brosseau⁵ 

[1]0000 0000 9039 7662, grid.7132.7, Department of Parasitology, Faculty of Medicine, Chiang Mai University, 50200, Chiang Mai, Thailand

[2]0000 0001 0944 049X, grid.9723.f, Department of Entomology, Faculty of Agriculture, Kasetsart University, 10900, Bangkok, Thailand

[3]0000 0001 0944 049X, grid.9723.f, Department of Entomology, Faculty of Agriculture, Kasetsart University, 10900, Bangkok, Thailand

[4]Department of Public Health and Malaria Control, International SOS, Jl. Kertajasa, Kuala Kencana, 99920, Papua, Indonesia

[5]0000 0001 0944 049X, grid.9723.f, Department of Zoology, Faculty of Science, Kasetsart University, 10900, Bangkok, Thailand

[6]0000 0001 2097 0141, grid.121334.6, HydroSciences Montpellier (HSM), Institut de Recherche pour le Développement (IRD), CNRS, Université Montpellier, Montpellier, France

关键词: Anopheles;    Barbirostris Complex;    ITS2;    multiplex PCR;    Thailand;   

DOI  :  10.1186/s13071-019-3494-8

来源: publisher

【 摘 要 】

BackgroundThe Barbirostris Complex comprises six formally described species that cannot be differentiated based on morphology alone. Out of these six species, two have been reported as putative malaria vectors, An. campestris and An. wejchoochotei. Five species are present in Thailand, An. barbirostris, An. campestris, An. dissidens, An. saeungae and An. wejchoochotei, while An. vanderwulpi occurs in Indonesia. As these species cannot be accurately differentiated by morphological characters, there is a crucial lack of information on their bionomics and role in the transmission of malaria and filariasis agents.ResultsFor differentiating the six species, an allele-specific amplification (AS-PCR) based on the second internal transcribed spacer (ITS2) sequence was developed. From 862 mosquitoes in the Barbirostris Complex collected in 23 provinces throughout Thailand, the AS-PCR was able to identify five species and its validation was undertaken on 185 specimens.ConclusionsThis multiplex-PCR assay is potentially able to definitely identify all six species of the Barbirostris Complex and was validated on five species present in Thailand.

【 授权许可】

CC BY