Stabilized tetraether lipids based particles guided prophyrins photodynamic therapy

Drug Delivery

Udo Bakowsky¹  Jarmila Jedelská¹  Boris Strehlow¹  Gihan Mahmoud²  Samia Mohamed Omar³  Marc Schneider⁴ 

[1]Department of Pharmaceutics and Biopharmaceutics, University of Marburg, Marburg, Germany

[2]Department of Pharmaceutics and Biopharmaceutics, University of Marburg, Marburg, Germany

[3]Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Helwan University, Cairo, Egypt

[4]Department of Pharmaceutics, Faculty of Pharmacy, Ahram Canadian University, Giza, Egypt

[5]Department of Pharmacy, Biopharmaceutics and Pharmaceutical Technology, Saarland University, Saarbrücken, German

关键词: Tetraether lipids;    liposomes;    photodynamic therapy;    protoporphyrin IX;    chick chorioallantoic membrane;   

DOI  :  10.1080/10717544.2018.1482970

来源: publisher

【 摘 要 】

Photodynamic therapy (PDT) that involves ergonomically delivered light in the presence of archetypical photosensitizer such as Protoporphyrin IX (PpIX) is a time-honored missile strategy in cancer therapeutics. Yet, the premature release of PpIX is one of the most abundant dilemma encounters the therapeutic outcomes of PDT due to associated toxicity and redistribution to serum proteins. In this study, ultrastable tetraether lipids (TELs) based liposomes were developed. PpIX molecules were identified to reside physically in the monolayer; thereby the inherent π-π stacking that leads to aggregation of PpIX in aqueous milieu was dramatically improved. TEL29.9 mol% and TEL62mol% based liposomes revealed PpIX sustained release diffusion pattern from spherical particles as confirmed by converged fitting to Baker & Lonsdale model. Stability in presence of human serum albumins, a key element for PDT accomplishment was emphasized. The epitome candidates were selected for vascular photodynamic (vPDT) in in-Ovo chick chorioallantoic membrane. Profoundly, TEL62mol% based liposomes proved to be the most effective liposomes that demonstrated localized effect within the irradiated area without eliciting quiescent vasculatures damages. Cellular photodynamic therapy (cPDT) revealed that various radiant exposure doses of 134, 202, 403 or 672 mJ.cm−2 could deliberately modulate the photo-responses of PpIX in TEL-liposomes.

【 授权许可】

CC BY