Rapid and homologous immunoassay for the detection of herbicide propisochlor in water

Food and Agricultural Immunology

Sergei A. Eremin¹  Yuan-Ming Sun²  Lan-Teng Wang²  Kai Zhou²  Yao Liu²  Zhen-Lin Xu²  Hong-Tao Lei²  Xin-An Huang³ 

[1] Faculty of Chemistry, MV Lomonosov Moscow State University, Moscow, Russi;Guangdong Provincial Key Laboratory of Food Quality and Safety, National-Local Joint Engineering Research Center for Processing and Safety Control of Livestock and Poultry Products, College of Food Science, South China Agricultural University, Guangzhou, People’s Republic of Chin;Tropical Medicine Institute and South China Chinese Medicine Collaborative Innovation Center, Guangzhou University of Chinese Medicine, Guangzhou, People’s Republic of Chin;

关键词: Propisochlor;    fluorescence polarization immunoassay;    pond water;    molecular modeling;   

DOI  :  10.1080/09540105.2017.1359499

来源: publisher

【 摘 要 】

The herbicide propisochlor can be introduced into the water cycle via agricultural activities, thus possibly leading to harmful ecological effects. It is significant for the development of a rapid method for propisochlor analysis in water. In this work, a rapid homogeneous immunoassay based on fluorescence polarization immunoassay (FPIA) was firstly developed for the determination of propisochlor in pond water. The developed FPIA assay can be completed within only 14 min; the 50% inhibition concentration (IC50) was 125.1 ng/mL and the limit of detection was 8.4 ng/mL. The recoveries of propisochlor in pond water ranged from 80.0% to 86.6%. A high performance liquid chromatography confirmation showed a good correlation with the developed FPIA (R2 = 0.9748). The developed FPIA exhibited satisfactory rapidity, high sensitivity and excellent specificity, meeting the standards of the screening assay of propisochlor in pond water on site. The molecular modeling showed that the alkoxyalkyl of chloroacetamide herbicides exerted important effects on the herbicide–antibody binding.

【 授权许可】

CC BY