An in vivo study of Hypericum perforatum in a niosomal topical drug delivery system

Drug Delivery

Omaima N. El-Gazayerly¹  Mohammed A. Ahmed¹  Abdulrhman Alsayari²  Amira Abdel Motaal³  Mohamed Ali⁴ 

[1] Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Cairo University, Cairo, Egyp;Department of Pharmacognosy, College of Pharmacy, King Khaled University, Abha, Kingdom of Saudi Arabia;Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo, Egypt;Department of Pharmacognosy, College of Pharmacy, King Khaled University, Abha, Kingdom of Saudi Arabia;Department of Research and Development, Atos Pharma, Cairo, Egypt;

关键词: Hypericum perforatum;    niosomes;    in vivo;    standardization;    wound;   

DOI  :  10.1080/10717544.2018.1431977

来源: publisher

【 摘 要 】

The active compounds present in Hypericum perforatum L. (Hypericaceae) include hyperforin, hypericins and flavonoids, which are assumed to be responsible for the activity of the extract in the treatment of wounds and scars. The present study aimed to incorporate H. perforatum extract standardized to a known content of phloroglucinols, naphthodianthrones and polyphenolic compounds into an effective transdermal drug delivery system capable of entrapping both lipophilic and hydrophilic constituents in the form of niosomal gels for wound treatment. An 80% ethanol extract (HE) was prepared on a pilot scale using DIG-MAZ. An HPLC-DAD holistic profile was established for HE and was standardized to contain 3.4 ± 4 rutin, 1.1 ± 3 chlorogenic acid, 0.5 ± 2 quercitrin, 2.8 ± 2 hyperforin, and 0.51 ± 3% w/w total hypericins. Niosomes were prepared using the modified reverse phase evaporation technique (REV). The wound healing effect of the gel was tested on 16 adult mongrel dogs. A significant decrease in the inflammatory cell count (18.4 ± 5.3) was recorded in the niosomal gel 1.5% NaCMC-treated group at the 7th day post wounding. It induced a marked regression in the inflammatory phase and enhanced the early beginning of the proliferative phase of wound healing. After 21 days, it showed complete re-epithelization, formation of new matrix fibers and significant reduction in the wound size, compared to the control and the Panthenol® 2% cream treated groups. This is the first study of H. perforatum in a niosomal topical drug delivery system.

【 授权许可】

CC BY