Application of an assay Cascade methodology for a deep preclinical characterization of polymeric nanoparticles as a treatment for gliomas

Drug Delivery

Marta Guerra-Rebollo¹  Jerónimo Blanco¹  Núria Rubio¹  Cristina Garrido¹  Irene Porcar²  Cristina Fornaguera²  Miguel Ángel Lázaro²  Ingrid Morera²  Anna Cascante³  Pau Brugada-Vilà³  Salvador Borrós⁴ 

[1] Institut de Química Avançada de Catalunya (IQAC-CSIC), Barcelona, Spain;Centro de Investigación Biomédica en Red en Bioingenierı´a, Biomateriales y Nanomedicina (CIBER-BBN), Barcelona, Spai;Sagetis-Biotech, Barcelona, Spain;Sagetis-Biotech, Barcelona, Spain;Grup d’Enginyera de Materials (GEMAT), Institut Químic de Sarrià, Universitat Ramon Llull, Barcelona, Spain;Sagetis-Biotech, Barcelona, Spain;Grup d’Enginyera de Materials (GEMAT), Institut Químic de Sarrià, Universitat Ramon Llull, Barcelona, Spain;Centro de Investigación Biomédica en Red en Bioingenierı´a, Biomateriales y Nanomedicina (CIBER-BBN), Barcelona, Spai;

关键词: NCL assay cascade protocol;    nanoparticle preclinical characterization;    research lab results translation;    polymeric nanoparticles;    paclitaxel;    glioblastoma multiforme;   

DOI  :  10.1080/10717544.2018.1436099

来源: publisher

【 摘 要 】

Glioblastoma multiforme (GBM) is the most devastating primary brain tumor due to its infiltrating and diffuse growth characteristics, a situation compounded by the lack of effective treatments. Currently, many efforts are being devoted to find novel formulations to treat this disease, specifically in the nanomedicine field. However, due to the lack of comprehensive characterization that leads to insufficient data on reproducibility, only a reduced number of nanomedicines have reached clinical phases. In this context, the aim of the present study was to use a cascade of assays that evaluate from physical-chemical and structural properties to biological characteristics, both in vitro and in vivo, and also to check the performance of nanoparticles for glioma therapy. An amphiphilic block copolymer, composed of polyester and poly(ethylene glycol; PEG) blocks, has been synthesized. Using a mixture of this copolymer and a polymer containing an active targeting moiety to the Blood Brain Barrier (BBB; Seq12 peptide), biocompatible and biodegradable polymeric nanoparticles have been prepared and extensively characterized. In vitro studies demonstrated that nanoparticles are safe for normal cells but cytotoxic for cancer cells. In vivo studies in mice demonstrated the ability of the Seq12 peptide to cross the BBB. Finally, in vivo efficacy studies using a human tumor model in SCID mice resulted in a significant 50% life-span increase, as compared with non-treated animals. Altogether, this assay cascade provided extensive pre-clinical characterization of our polymeric nanoparticles, now ready for clinical evaluation.

【 授权许可】

CC BY