Effective biodegradation of chicken feather waste by co-cultivation of keratinase producing strains

Microbial Cell Factories

Juan Zhang¹  Xinzhe Mao¹  Guocheng Du¹  Zheng Peng¹  Jian Chen² 

[1] 0000 0001 0708 1323, grid.258151.a, School of Biotechnology, Jiangnan University, 1800 Lihu Road, 214122, Wuxi, China;0000 0001 0708 1323, grid.258151.a, Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, 1800 Lihu Road, 214122, Wuxi, China;0000 0001 0708 1323, grid.258151.a, School of Biotechnology, Jiangnan University, 1800 Lihu Road, 214122, Wuxi, China;0000 0001 0708 1323, grid.258151.a, National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, 1800 Lihu Road, 214122, Wuxi, China;

关键词: Chicken feather waste;    Biodegradation;    Cocultivation;    Laboratory scale fermenter;    Hydrolysate;   

DOI  :  10.1186/s12934-019-1134-9

来源: publisher

【 摘 要 】

BackgroundChicken feather, a byproduct of poultry-processing industries, are considered a potential high-quality protein supplement owing to their crude protein content of more than 85%. Nonetheless, chicken feathers have been classified as waste because of the lack of effective recycling methods. In our previous studies, Bacillus licheniformis BBE11-1 and Stenotrophomonas maltophilia BBE11-1 have been shown to have feather-degrading capabilities in the qualitative phase. To efficiently recycle chicken feather waste, in this study, we investigated the characteristics of feather degradation by B. licheniformis BBE11-1 and S. maltophilia BBE11-1. In addition, in an analysis of the respective advantages of the two degradation systems, cocultivation was found to improve the efficiency of chicken feather waste degradation.ResultsB. licheniformis BBE11-1 and S. maltophilia BBE11-1 were used to degrade 50 g/L chicken feather waste in batches, and the degradation rates were 35.4% and 22.8% in 96 h, respectively. The degradation rate of the coculture system reached 55.2% because of higher keratinase and protease activities. Furthermore, cocultivation was conducted in a 3 L fermenter by integrating dissolved oxygen control and a two-stage temperature control strategy. Thus, the degradation rate was greatly increased to 81.8%, and the conversion rate was 70.0% in 48 h. The hydrolysates exhibited antioxidant activity and contained large quantities of amino acids (895.89 mg/L) and soluble peptides.ConclusionsCocultivation of B. licheniformis BBE11-1 and S. maltophilia BBE11-1 can efficiently degrade 50 g/L chicken feather waste and produce large amounts of amino acids and antioxidant substances at a conversion rate of 70.0%.

【 授权许可】

CC BY