A novel anti-platelet aggregation target of chinensinaphthol methyl ether and neojusticin B obtained from Rostellularia procumbens (L.) Nees

Journal of Enzyme Inhibition and Medicinal Chemistry

Wenping Xiao¹  Yuan Sun¹  Weichen Xiong¹  Songtao Wu¹  Pengfei Hao¹  Zhoutao Xie¹  Yanfang Yang²  Zhongzhu Ai²  Hezhen Wu²  Bo Liu² 

[1] Faculty of Pharmacy, Hubei University of Chinese Medicine, Wuhan, China;Faculty of Pharmacy, Hubei University of Chinese Medicine, Wuhan, China;Key Laboratory of Traditional Chinese Medicine Resources and Chemistry of Hubei Province, Wuhan, China;Collaborative Innovation Center of Traditional Chinese Medicine of New Products for Geriatrics Hubei Province, Wuhan, Chin;

关键词: Chinensinaphthol methyl ether;    neojusticin B;    β;    platelet aggregation;    gene chip;    network pharmacology;    Prometheus NT.48;    microscale thermophoresis;   

DOI  :  10.1080/14756366.2019.1609468

来源: publisher

【 摘 要 】

This study explored the possible bioactive ingredients and target protein of Rostellularia procumbens (L.) Nees. The results of optical turbidimetry revealed that the ethyl acetate extraction obtained from R. procumbens (L.) Nees could inhibit platelet aggregation. Gene chip was used to investigate differentially expressed genes. According to the results of the gene chip, the targets of compounds isolated from the ethyl acetate extraction were predicted by network pharmacology. Computational studies revealed that chinensinaphthol methyl ether and neojusticin B may target the integrin αIIbβ3 protein. The results of Prometheus NT.48 and microscale thermophoresis suggested that the molecular interactions between the two compounds with purified integrin αIIbβ3 protein in the optimal test conditions were coherent with the docking results. To our best knowledge, this is the first report to state that chinensinaphthol methyl ether and neojusticin B target the integrin αIIbβ3 protein.

【 授权许可】

CC BY