Proteomics analysis reveals heat shock proteins involved in caprine parainfluenza virus type 3 infection

BMC Veterinary Research

Maojun Liu¹  Leilei Yang¹  Wenliang Li¹  Min Sun¹  Wenwen Zhang¹  Jizong Li²  Chunyan Zhong³  Fang Xiao³  Zheng Liao³  Li Mao⁴  Xing Zhu⁵  Xinqin Ji⁵ 

[1] 0000 0001 0017 5204, grid.454840.9, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, 210014, Nanjing, China;0000 0001 0017 5204, grid.454840.9, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, 210014, Nanjing, China;0000 0004 1763 3680, grid.410747.1, School of Pharmacy, Linyi University, 276000, Linyi, China;0000 0001 0017 5204, grid.454840.9, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, 210014, Nanjing, China;0000 0004 1804 268X, grid.443382.a, College of Animal Science, Guizhou University, 550025, Guiyang, China;0000 0001 0017 5204, grid.454840.9, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, 210014, Nanjing, China;Key Lab of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, 210014, Nanjing, China;0000 0004 1804 268X, grid.443382.a, College of Animal Science, Guizhou University, 550025, Guiyang, China;

关键词: Caprine parainfluenza virus type 3;    Madin-Darby bovine kidney cells;    Proteomic analysis;    iTRAQ;    LC-MS/MS;   

DOI  :  10.1186/s12917-019-1897-6

来源: publisher

【 摘 要 】

BackgroundCaprine parainfluenza virus type 3 (CPIV3) is major pathogen of goat herds causing serious respiratory tract disease and economic losses to the goat industry in China. We analyzed the differential proteomics of CPIV3-infected Madin-Darby bovine kidney (MDBK) cells using quantitative iTRAQ coupled LC-MS/MS. In addition, four DEPs were validated by qRT-PCR and western blot analysis.ResultsQuantitative proteomics analysis revealed 163 differentially expressed proteins (DEPs) between CPIV3-infected and mock-infected groups (p-value < 0.05 and fold change > 1.2), among which 91 were down-regulated and 72 were up-regulated. Gene ontology (GO) analysis showed that these DEPs were involved in molecular functions, cellular components and biological processes. Biological functions in which the DEPs were involved in included diseases, genetic information processing, metabolism, environmental information processing, cellular processes, and organismal systems. STRING analysis revealed that four heat shock proteins (HSPs) included HSPA5, HSPA1B, HSP90B1 and HSPA6 may be associated with proliferation of CPIV3 in MDBK cells. qRT-PCR and western blot analysis showed that the selected HSPs were identical to the quantitative proteomics data.ConclusionTo our knowledge, this is the first report of the proteomic changes in MDBK cells after CPIV3 infection.

【 授权许可】

CC BY