Food and Agricultural Immunology | |
Homologous ELISA for detection of prednisolone in human serum | |
T. G. Shrivastav1  Dinesh Kumar2  Harpal Singh3  | |
[1] Immuno, Isotope and Nano-technology Laboratory, Department of Reproductive Biomedicine, The National Institute of Health and Family Welfare (NIHFW), New Delhi, Indi;Immuno, Isotope and Nano-technology Laboratory, Department of Reproductive Biomedicine, The National Institute of Health and Family Welfare (NIHFW), New Delhi, Indi;Indian Institute of Technology Delhi (IIT-D), New Delhi, Indi;All India Institute of Medical Sciences Delhi (AIIMS-D), New Delhi, Indi;Indian Institute of Technology Delhi (IIT-D), New Delhi, Indi;All India Institute of Medical Sciences Delhi (AIIMS-D), New Delhi, Indi; | |
关键词: Prednisolone; immunogen; antibody; horseradish peroxidase; ELISA; | |
DOI : 10.1080/09540105.2017.1376184 | |
来源: publisher | |
【 摘 要 】
An enzyme-linked immunosorbent assay to measure prednisolone have been developed. An antibody was raised in rabbits using prednisolone-21-hemisuccinate (PSL-21-HS) conjugated to bovine serum albumin. Similarly, PSL-21-HS was conjugated to horseradish peroxidase to prepare enzyme conjugate. The developed assay has been validated for sensitivity and effective displacement at 50% of the assay were 0.078 and 2.64 ng/mL, respectively. This assay showed cross-reaction with only 4 steroids – i.e. progesterone 1.76%, 17α-OH progesterone 5.89%, cortisol 7.69% and prednisone 1.13%, out of 55 analogous steroids. The percent recovery of prednisolone from the exogenously spiked human serum pools was in the range of 94.84–100.17%. The intra-assay and inter-assay coefficients of variation ranged from 5.79% to 8.00% and from 3.23% to 8.63%, respectively. The serum prednisolone values obtained by this method correlated well with the commercially available kit and found to be 0.93.
【 授权许可】
CC BY
【 预 览 】
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