Development of an ic-ELISA and immunochromatographic strip for detection of sparfloxacin in honey

Food and Agricultural Immunology

Jingjing Li¹  Hui Nie¹  Aimin Zhi¹  Guochao Jia²  Lianzhong Ai² 

[1] Chemical Technology and Food Science College, Zhengzhou Institute of Engineering and Technology, Zhengzhou, Henan, People’s Republic of Chin;School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai, People’s Republic of Chin;

关键词: Sparfloxacin;    monoclonal antibody;    ic-ELISA;    ICA strip;    honey;   

DOI  :  10.1080/09540105.2017.1364706

来源: publisher

【 摘 要 】

In this study, a sensitive monoclonal antibody (mAb) 3D5 against sparfloxacin (SPFX) was generated. Based on the mAb 3D5, an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) method was developed for the detection of SPFX in honey. The half maximal inhibitory concentration and limit of detection for the ic-ELISA method were 0.12 and 0.02 ng/mL, respectively. The experiments showed a negligible cross-reaction with other drugs. The average recovery rates for spiked SPFX honey extracts ranged from 90% to 101%, indicating an accepted accuracy. Furthermore, a lateral-flow immunochromatographic assay strip method with a cut-off value of 2 ng/mL was developed. Therefore, both of the developed methods are suitable for future use as rapid screening methods to detect and control the content of SPFX residues in honey samples.

【 授权许可】

CC BY