Estimating absolute indoor density of Aedes aegypti using removal sampling

Parasites & Vectors

Mike Dunbar¹  Gonzalo Vazquez-Prokopec¹  Audrey Lenhart²  Guadalupe Ayora-Talavera³  Norma Pavía-Ruz³  Azael Cohuo-Rodríguez⁴  Pablo Manrique-Saide⁴  Anuar Medina-Barreiro⁴  Edgar Koyoc-Cardeña⁴ 

[1] 0000 0001 0941 6502, grid.189967.8, Department of Environmental Sciences, Emory University, Atlanta, GA, USA;0000 0001 2163 0069, grid.416738.f, Entomology Branch, Division of Parasitic Diseases and Malaria, Center for Global Health, Centers for Disease Control and Prevention, Atlanta, GA, USA;0000 0001 2188 7788, grid.412864.d, Centro de Investigaciones Regionales “Dr. Hideyo Noguchi”, Universidad Autónoma de Yucatán, Mérida, Yucatán, Mexico;0000 0001 2188 7788, grid.412864.d, Unidad Colaborativa de Bioensayos Entomológicos, Campus de Ciencias. Biológicas y Agropecuarias, Universidad Autónoma de Yucatán, Mérida, Yucatán, Mexico;

关键词: Sampling;    Entomology;    Population abundance;   

DOI  :  10.1186/s13071-019-3503-y

来源: publisher

【 摘 要 】

BackgroundQuantification of adult Aedes aegypti abundance indoors has relied on estimates of relative density (e.g. number of adults per unit of sampling or time), most commonly using traps or timed collections using aspirators. The lack of estimates of the sensitivity of collections and lack of a numerical association between relative and the absolute density of adult Ae. aegypti represent a significant gap in vector surveillance. Here, we describe the use of sequential removal sampling to estimate absolute numbers of indoor resting Ae. aegypti and to calculate calibration coefficients for timed Prokopack aspirator collections in the city of Merida, Yucatan State, Mexico. The study was performed in 200 houses that were selected based on recent occurrence of Aedes-borne viral illness in residents. Removal sampling occurred in 10-minute sampling rounds performed sequentially until no Ae. aegypti adult was collected for 3 hours or over 2 consecutive 10-minute periods.ResultsA total of 3439 Ae. aegypti were collected. The sensitivity of detection of positive houses in the first sampling round was 82.5% for any adult Ae. aegypti, 78.5% for females, 75.5% for males and 73.3% for blood-fed females. The total number of Ae. aegypti per house was on average ~5 times higher than numbers collected for the first sampling round. There was a positive linear relationship between the relative density of Ae. aegypti collected during the first 10-min round and the absolute density for all adult metrics. Coefficients from the linear regression were used to calibrate numbers from 10-min collections into estimates of absolute indoor Ae. aegypti density for all adults, females and males.ConclusionsExhaustive removal sampling represents a promising method for quantification of absolute indoor Ae. aegypti density, leading to improved entomological estimates of mosquito distribution, a key measure in the assessments of the risk pathogen transmission, disease modeling and the evaluation of vector control interventions.

【 授权许可】

CC BY