Inhibition of microRNA-183 expression resists human umbilical vascular endothelial cells injury by upregulating expression of IRS1

Drug Delivery

Bo Yu¹  Dandan Liu¹  Yingying Zhang¹  Yefei Zhan² 

[1] Department of Cardiology, 2nd Affiliated Hospital of Harbin Medical University, and the Key Laboratory of Myocardial Ischemia, Chinese Ministry of Education, Harbin, People’s Republic of China;Department of Intensive Care Unit, Ningbo No 2 Hospital, Ningbo, People’s Republic of Chin;

关键词: MicroRNA-183;    IRS1;    human umbilical vascular endothelial cells;    oxidized low-density lipoprotein;    cell injury;   

DOI  :  10.1080/10717544.2019.1628117

来源: publisher

【 摘 要 】

Our study aims to investigate the effect of microRNA-183 (miR-183) on human umbilical vascular endothelial cells (HUVECs) injury by targeting IRS1. HUVECs injury was induced by oxidized low-density lipoprotein (ox-LDL). HUVECs were grouped so as to explore the role of ox-LDL and miR-183 in HUVECs injury, with the expression of miR-183 and IRS1 detected. Additionally, the related factors of oxidative stress and inflammation, as well as angiogenesis ability, proliferation, cell cycle, apoptosis, invasion, and migration abilities were also measured. Ox-LDL treatment could decrease the activity of HUVECs, increase the level of oxidative stress and inflammation, and induce the HUVECs injury. miR-183 could inhibit the expression of IRS1. The inhibition of miR-183 expression in ox-LDL-induced HUVECs injury could enhance cell activity, inhibit inflammatory level, and thus resist cell injury. Low expression of IRS1 could reverse the inhibition of miR-183 on HUVECs injury. This study highlights that inhibition of miR-183 expression may resist HUVECs injury by upregulating expression of IRS1.

【 授权许可】

CC BY