Isolation of a 3-hydroxypyridine degrading bacterium, Agrobacterium sp. DW-1, and its proposed degradation pathway

AMB Express

Lizhong Guo¹  Shuxue Zhao¹  Hao Yu¹  Chunhui Hu²  Kuiran Li³ 

[1] 0000 0000 9526 6338, grid.412608.9, Shandong Provincial Key Laboratory of Applied Mycology, College of Life Sciences, Qingdao Agricultural University, 700 Changcheng Road, Chengyang District, 266109, Qingdao, Shandong Province, People’s Republic of China;0000 0000 9526 6338, grid.412608.9, Shandong Provincial Key Laboratory of Applied Mycology, College of Life Sciences, Qingdao Agricultural University, 700 Changcheng Road, Chengyang District, 266109, Qingdao, Shandong Province, People’s Republic of China;0000 0001 2152 3263, grid.4422.0, Key Laboratory of Marine Environmental Science and Ecology, Ministry of Education, Ocean University of China, 238 Songling Road, Laoshan District, 266100, Qingdao, Shandong Province, People’s Republic of China;0000 0001 2152 3263, grid.4422.0, Key Laboratory of Marine Environmental Science and Ecology, Ministry of Education, Ocean University of China, 238 Songling Road, Laoshan District, 266100, Qingdao, Shandong Province, People’s Republic of China;

关键词: 3-Hydroxypyridine;    Microbial degradation;    Agrobacterium;    Pathway;    2,5-Dihydroxypyridine;   

DOI  :  10.1186/s13568-019-0782-9

来源: publisher

【 摘 要 】

A 3-hydroxypyridine degrading bacterium, designated strain DW-1, was isolated from petroleum contaminated soil in Liao River China. 16S rRNA-based phylogenetic analysis indicates that strain DW-1 belongs to genus Agrobacterium. The optimal cultivation temperature and pH for strain DW-1 with 3-hydroxypyridine were 30 °C and 8.0, respectively. Under optimal conditions, strain DW-1 could completely degrade up to 1500 mg/L of 3-hydroxypyridine in 66 h. The 3-hydroxypyridine degradation pathway of strain DW-1 was suggested by HPLC and LC–MS analysis. The first reaction of 3-hydroxypyridine degradation in strain DW-1 was α-hydroxylation so that the major metabolite 2,5-dihydroxypyridine was produced, and then 2,5-dihydroxypyridine was transformed by a Fe2+-dependent dioxygenase to form N-formylmaleamic acid. N-Formylmaleamic acid will be transformed to maleic acid and fumaric acid through maleamic acid. This is the first report of the 3-hydroxypyridine degradation pathway and the utilization of 3-hydroxypyridine by a Agrobacterium sp. It may be potentially used for the bioremediation of environments polluted with 3-hydroxypyridine.

【 授权许可】

CC BY