Cortical anchoring of the microtubule cytoskeleton is essential for neuron polarity

eLife

AF Maarten Altelaar¹  Eric van Leen²  Robbelien Kooistra²  Liu He²  Anna van Regteren Altena²  Martin Harterink²  Sybren Portegies²  Bart de Haan²  Ellen Oudejans²  Riccardo Stucchi³  Casper C Hoogenraad⁴  Stefan Wieser⁵  Johannes Ziegler⁵  Michael Krieg⁶  Ravi Das⁶ 

[1] Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, Netherlands;Cell Biology, Neurobiology and Biophysics, Department of Biology, Faculty of Science, Utrecht University, Utrecht, Netherlands;Cell Biology, Neurobiology and Biophysics, Department of Biology, Faculty of Science, Utrecht University, Utrecht, Netherlands;Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, Netherlands;Cell Biology, Neurobiology and Biophysics, Department of Biology, Faculty of Science, Utrecht University, Utrecht, Netherlands;Department of Neuroscience, Genentech, Inc, South San Francisco, United States;Fast live-cell superresolution microscopy, ICFO-Institut de Ciencies Fotoniques, The Barcelona Institute of Science and Technology, Barcelona, Spain;Neurophotonics and Mechanical Systems Biology, ICFO-Institut de Ciencies Fotoniques, The Barcelona Institute of Science and Technology, Barcelona, Spain;

关键词: neuron polarity;    microtubule;    kinesin;    CRMP;    Ankyrin;    C. elegans;   

DOI  :  10.7554/eLife.55111

来源: publisher

【 摘 要 】

The development of a polarized neuron relies on the selective transport of proteins to axons and dendrites. Although it is well known that the microtubule cytoskeleton has a central role in establishing neuronal polarity, how its specific organization is established and maintained is poorly understood. Using the in vivo model system Caenorhabditis elegans, we found that the highly conserved UNC-119 protein provides a link between the membrane-associated Ankyrin (UNC-44) and the microtubule-associated CRMP (UNC-33). Together they form a periodic membrane-associated complex that anchors axonal and dendritic microtubule bundles to the cortex. This anchoring is critical to maintain microtubule organization by opposing kinesin-1 powered microtubule sliding. Disturbing this molecular complex alters neuronal polarity and causes strong developmental defects of the nervous system leading to severely paralyzed animals.

【 授权许可】

CC BY