Genotyping of Clostridium perfringens Isolates from Domestic Livestock in Saudi Arabia

BioMed Research International

Mohammed Z. Aljulaifi¹  Salah Eldin H. Babiker¹  Ebtesam M. Al-Olayan²  Sawsan A. Omer²  Abdulaziz N. Alagaili³  Osama B. Mohammed³ 

[1] Department of Veterinary Laboratories, Ministry of Agriculture, King Saud University, Riyadh, Saudi Arabia, ksu.edu.sa;Department of Zoology, College of Science, King Saud University, University Centre for Women Students, P. O. Box 22452, Riyadh 11495, Saudi Arabia, ksu.edu.sa;KSU Mammals Research Chair, Department of Zoology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia, ksu.edu.sa;

DOI  :  10.1155/2020/9035341

来源: publisher

【 摘 要 】

The present study was undertaken to confirm the genetic identity of Clostridium perfringens isolates from domestic livestock in Saudi Arabia and to characterize the genes encoding to alpha, beta, epsilon, and iota (α-, β-, ε-, and ι-) toxins. C. perfringens were confirmed in 104 out of 136 isolates on multiplex polymerase chain reaction using specific primers amplifying genes related to toxins produced by C. perfringens. Genes encoding α-toxins were detected in 104 samples. Of the isolates, 80.8% were diagnosed as type A, 15.4% as type D, 2.9% as type C, and 0.96% as type B. None of the isolates has genes encoding iota (ι-) toxin. All isolates investigated yielded enterotoxin (cpe) products and none yielded β2 (cpb2-toxin) or NetB products. PLC gene sequences encoding α-toxin showed >96.7% similarity. Isolates which had α-toxins as well as enterotoxin (cpe) are regarded as type F. Phylogenetic analysis using maximum likelihood analysis yielded two clades, and the majority of the isolates were in one group while only two isolates clustered on the second clade. Within the Kingdom of Saudi Arabia strains, 54 variable positions and 23 polymorphic amino acids were noticed. Isolates with ε- and β-toxins were variable and were found to be close to those published for C. perfringens. ETX gene sequences encoding ε-toxins were found to be related to CPE sequences.

【 授权许可】

CC BY