Effect of Turmeric Turmerin Curcumin on Ca2+, Na/K+Atpases in Concanavalin A-Stimulated Human Blood Mononuclear Cells

International Journal of Molecular Sciences

Hari H. P. Cohly²  Maheshwara-Rajeswara Rao²  Vijaya K. Kanji²  Babu Patlolla¹  Anelle Taylor²  Melanie T. Wilson²  Michael F. Angel² 

[1] ³Department of Biology, Alcorn State University, Lorman, MS, 39096;¹Departments of Surgery and ²Medicine, University of Mississippi Medical Center, Jackson, MS, 39217-4505, E-mail:

关键词: Turmeric;    turmerin;    curcumin;    Ca²⁺ATPase;    Na/K⁺ATPase;   

DOI  :  10.3390/i4020034

来源: mdpi

【 摘 要 】

Ion transport enzymes may play an important role in T cell activation. This study investigates the role of turmeric and its individual components, turmerin-and curcumin-on Ca²⁺ and Na/K⁺ adenosine triphosphatases (ATPase) in the course of T cell activation. Concanavalin A (Con A) stimulated human blood mononuclear T cell proliferation paradigm was investigated for 3, 5 and 7 day periods with different concentrations of turmeric, curcumin and turmerin.Con A-stimulated cells treated with turmeric (250, 50, 5 µg/ml) for 3 and 5 days inhibited ATPase levels when compared to base levels obtained by cells in media alone.At day 7, there was a 3-fold increase for Ca²⁺ATPase levels and a 2-fold increase for Na/K⁺ATPase.Curcumin (250, 50, 5 µg/ml) showed the same pattern for ATPase activity as turmeric at 3 and 5 days with a 2-fold increase at day 7.Turmerin (2500, 1250, 250, 25 ng/ml) for Na/K⁺ ATPase activity showed an increase at day 3, a decrease on day 5, and a 2-fold increase on day 7.Ca²⁺ ATPase activity in the presence of turmerin showed an increase in ATPase levels at day 3 (except at 2500ng/ml where it decreased) and a decrease in day 5 (except at 25 ng/ml where it increased).Turmeric and curcumin generally inhibited Ca²⁺ATPase and Na/K⁺ATPases in early (day 3) and intermediate (day 5) stages of mitogen stimulation.However, the effect after 7 days incubation for turmeric, curcumin and turmerin showed a marked increase up to three fold.

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