Atomic Force Microscope Imaging of the Aggregation of Mouse Immunoglobulin G Molecules

Molecules

Jiye Cai¹  Yao Chen²  Qingcai Xu²  Yong Chen²  Tao Zhao²  Xiaoyan Wang² 

[1] id="af1-molecules-08-00086">Department of Chemistry, Jinan University, Guangzhou, 510632, China; Tel. (+86) [phone], Fax (+86) (020)8522-62

关键词: Mouse immunoglobulin G;    self-assembly;    aggregation;    atomic force microscope;   

DOI  :  10.3390/80100086

来源: mdpi

【 摘 要 】

Mouse immunoglobulin G (Ig G1 and the mixture of Ig G1 and Ig G2) deposited on mica were imaged with an atomic force microscope at room temperature and ambient pressure. At a concentration around 1.0mg/L, the molecules were well dispersed. 2~3 days after sample preparation, both Ig G1 and the mixture could self-assemble into different shapes and further form some types of local-ordered toroidal aggregations (monotoroidal, intercrossed toroidal, concentric toroidal, etc.). The number of monomers was not identical in the different toroidal aggregations but in a same circle, the shapes of polymer self-assembled by several monomolecules were found to be almost the same. There was difference between the aggregation behavior of Ig G1 and the mixture. The mechanism of Ig G molecule aggregation was ascribed to the “Y” shape and loops structure of Ig G molecule.

【 授权许可】

Unknown   
© 2003 by MDPI (http://www.mdpi.org).