The Influence of the Enzyme Membrane Thickness on the Response of Amperometric Biosensors

Sensors

Romas Baronas¹  Feliksas Ivanauskas¹ 

[1] Faculty of Mathematics and Informatics, Vilnius University, Naugarduko 24, 2600 Vilnius, Lithuania

关键词: Amperometric biosensor;    enzyme membrane;    diffusion;    modelling;    simulation;   

DOI  :  10.3390/s30700248

来源: mdpi

【 摘 要 】

A mathematical model of amperometric biosensors has been  developed.  The model is based on non-stationary diffusion equations containing a non-linear term related to Michaelis-Menten kinetics of the enzymatic reaction. Using digital simulation, the influence of the thickness of enzyme membrane on the biosensor  response  was  investigated.  The digital simulation of the biosensor operation showed the non-monotonous change of the maximal biosensor current versus the membrane thickness at the various maximal enzymatic rates. Digital simulation was carried out using the finite difference technique. Results of the numerical simulation was compared with known analytical solutions. This paper presents a framework for selection of the membrane thickness, ensuring the sufficiently stable sensitivity of a biosensor in a required range of the maximal enzymatic rate.

【 授权许可】

Unknown   
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