Induced Mitogenic Activity in AML-12 Mouse Hepatocytes Exposed to Low-dose Ultra-Wideband Electromagnetic Radiation

International Journal of Environmental Research and Public Health

W. C. Dorsey¹  B. D. Ford³  L. Roane¹  D. T. Haynie² 

[1] Wildlife Biology Unit, Grambling State University, Grambling, LA. USA.;Biomedical Engineering & Physics, Institute for Micromanufacturing, Louisiana Tech University, Ruston, LA. USA.;Department of Anatomy and Neurobiology, Morehouse School of Medicine, Atlanta, GA. USA.

关键词: Ultra-wideband radiation;    nanopulse;    mitogenic activity;    mouse hepatocytes;   

DOI  :  10.3390/ijerph2005010024

来源: mdpi

【 摘 要 】

Ultra–wideband (UWB) technology has increased with the use of various civilian and military applications. In the present study, we hypothesized that low-dose UWB electromagnetic radiation (UWBR) could elicit a mitogenic effect in AML-12 mouse hepatocytes, in vitro. To test this hypothesis, we exposed AML-12 mouse hepatocytes, to UWBR in a specially constructed gigahertz transverse electromagnetic mode (GTEM) cell. Cells were exposed to UWBR for 2 h at a temperature of 23°C, a pulse width of 10 ns, a repetition rate of 1 kHz, and field strength of 5–20 kV/m. UWB pulses were triggered by an external pulse generator for UWBR exposure but were not triggered for the sham exposure. We performed an MTT Assay to assess cell viability for UWBR-treated and sham-exposed hepatocytes. Data from viability studies indicated a time-related increase in hepatocytes at time intervals from 8–24 h post exposure. UWBR exerted a statistically significant (p < 0.05) dose-dependent response in cell viability in both serum-treated and serum free medium (SFM) -treated hepatocytes. Western blot analysis of hepatocyte lysates demonstrated that cyclin A protein was induced in hepatocytes, suggesting that increased MTT activity after UWBR exposure was due to cell proliferation. This study indicates that UWBR has a mitogenic effect on AML-12 mouse hepatocytes and implicates a possible role for UWBR in hepatocarcinoma.

【 授权许可】

CC BY   
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