Supramolecular Complex Formation by β-Cyclodextrin and Ferrocenylnaphthalene Diimide-intercalated Double Stranded DNA and Improved Electrochemical Gene Detection

Molecules

Shinobu Sato¹  Takahiko Nojima¹  Michinori Waki¹ 

[1] id="af1-molecules-10-00693">Department of Materials Science, Kyushu Institute of Technology, 1-1 Sensui-cho, Tobata-ku, Kitakyushu-shi, Fukuoka, 804-8550, Japan. Phone&Fax:[phone]

关键词: Ferrocenylnaphthalene diimide;    β-cyclodextrin (CD);    DNA;    electrochemical detection;    supramolecular complex;   

DOI  :  10.3390/10060693

来源: mdpi

【 摘 要 】

Ferrocenylnaphthalene diimide 1 can bind to double stranded DNA (dsDNA) by the threading intercalation mode and the resulting complex was stabilized further by β-cyclodextrin (CD) by forming a supramolecular complex. These complex formation processes were studied by spectroscopic, viscometric, and electrochemical means in the absence or presence of β-CD. Quantitative analysis by quartz crystal microbalance (QCM) and electrochemical experiments strongly suggested a 2:1 binding stoichiometry for β-CD to 1 threading-intercalated to the dsDNA-immobilized electrode. Owing to this supramolecular complex formation, electrochemical DNA detection based on 1 was improved considerably.

【 授权许可】

Unknown   
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