Pulsed Amperometry for Anti-fouling of Boron-doped Diamond in Electroanalysis of β-Agonists: Application to Flow Injection for Pharmaceutical Analysis

Sensors

Chanpen Karuwan²  Thitirat Mantim²  Patcharin Chaisuwan²  Prapin Wilairat²  Kate Grudpan³  Piyada Jittangprasert²  Yasuaki Einaga⁴  Orawon Chailapakul⁵  Leena Suntornsuk¹  Oraphan Anurukvorakun¹ 

[1] Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Mahidol University, Thailand;Flow Innovation-Research for Science and Technology Laboratories (FIRST Labs.), Faculty of Science, Mahidol University, Rama 6 Road, Bangkok, 10400 Thailand;Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai, Thailand;Department of Chemistry, Faculty of Science and Technology, Keio University, Japan;Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok, Thailand

关键词: boron-doped diamond;    pulsed amperometry;    β-agonists;    flow injection;   

DOI  :  10.3390/s6121837

来源: mdpi

【 摘 要 】

This work presents the construction and application of boron-doped diamond (BDD) thin film electrode as sensor for the determination of three β-agonists, viz. salbutamol, terbutaline and clenbuterol. Although well-known as a chemically inert material, BDD film however shows fouling in detection of these compounds using fixed-potential mode amperometry. A suitable waveform for pulsed amperometric detection (PAD) was developed and used to determine the agonist compounds. It was seen that the developed PAD significantly refreshed the BDD surface for long-term detection in flow injection analysis. Linear working ranges were 0.5-100 μM, 1.0-100 μM and 0.5-50 μM for salbutamol, terbutaline and clenbuterol, respectively. The developed PAD-BDD system was applied to successfully determine salbutamol and terbutaline in commercial pharmaceutical products. The methods were validated with a capillary electrophoresis method.

【 授权许可】

Unknown   
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