Development of a DNA Sensor Based on Alkanethiol Self-Assembled Monolayer-Modified Electrodes

Sensors

Óscar A. Loaiza¹  Susana Campuzano¹  Mar໚ López-Berlanga¹  Mar໚ Pedrero¹ 

[1] id="af1-sensors-05-00344">Departamento de Química Analítica, Facultad de Ciencias Químicas, Universidad Complutense de Madrid, E-28040 - Madrid, Spai

关键词: Calf thymus DNA;    self-assembled monolayers;    hybridization;    denaturation;    methylene blue;   

DOI  :  10.3390/s5060344

来源: mdpi

【 摘 要 】

An electrochemical DNA biosensor based on recognition of double or single stranded DNA (ds-DNA/ss-DNA) immobilised on a self-assembled modified gold electrode is presented for denaturalisation and hybridisation detection. DNA is covalently bond on a self assembled 3-mercaptopropionic acid monolayer by using water soluble N-3-(dimethylaminopropyl)-N prime;ethylcarbodiimide hydrochloride (EDC) and N-hydroxisulfosuccinimide (NHSS) as linkers. The interaction between the immobilised DNA and methylene blue (MB) is investigated using square wave voltammetry (SWV). The increase or diminution of peak currents of the MB upon the hybridisation or denaturalisation event at the modified electrode surface is studied.

【 授权许可】

Unknown   
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