Elimination Voltammetry with Linear Scan as a New Detection Method for DNA Sensors

Sensors

Libuse Trnkova³  Frantisek Jelen¹  Jitka Petrlova²  Vojtech Adam²  David Potesil² 

[1] Institute of Biophysics, Academy of Sciences of the Czech Republic, Kralovopolska 135, 612 65 Brno, Czech Republic;Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry, Zemedelska 1, 613 00 Brno, Czech Republic;Department of Theoretical and Physical Chemistry, Masaryk University, Faculty of Science, Kotlarska 2, 611 37 Brno, Czech Republic

关键词: Oligodeoxynucleotide;    DNA biosensor;    linear sweep voltammetry;    elimination voltammetry with linear scan;    reduction of adenine and cytosine;    mercury electrode;    sequence of nucleotides;   

DOI  :  10.3390/s5060448

来源: mdpi

【 摘 要 】

The paper describes successful coupling of adsorptive transfer stripping (AdTS) and elimination voltammetry with linear scan (EVLS) for the resolution of reduction signals of cytosine (C) and adenine (A) residues in hetero-oligodeoxynucleotides (ODNs). Short ODNs (9-mers and 20-mers) were adsorbed from a small volume on a hanging mercury drop electrode (HMDE). After washing of the ODN-modified electrode by water and its transferring to an electrochemical cell, voltammetric curves were measured. The AdTS EVLS was able to determine of C/A ratio of ODNs through the elimination function conserving the diffusion current component and eliminating kinetic and charging current components. This function, which provides the elimination signal in a peak-counterpeak form, increased the current sensitivity for A and C resolution, and for the recognition of bases sequences in ODN chains. Optimal conditions of elimination experiments such as pH, time of adsorption, and scan rate were found. The combination of EVLS with AdTS procedure can be considered as a new detection method in a DNA sensor.

【 授权许可】

Unknown   
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