Brain Tissue Oxygen: In Vivo Monitoring with Carbon Paste Electrodes

Sensors

Fiachra B. Bolger¹ 

[1] id="af1-sensors-05-00473">Department of Pharmacology, Conway Institute of Biomolecular & Biomedical Research, University College Dublin, Belfield, Dublin 4, Irela

关键词: In-vivo voltammetry;    cerebral blood flow;    hypoxia and hyperoxia;    neuronal activation;    chloral hydrate anaesthesia;    acetazolamide;   

DOI  :  10.3390/s5110473

来源: mdpi

【 摘 要 】

In this communication we review selected experiments involving the use of carbon paste electrodes (CPEs) to monitor and measure brain tissue O₂ levels in awake freely-moving animals. Simultaneous measurements of rCBF were performed using the H₂ clearance technique. Voltammetric techniques used include both differential pulse (O₂) and constant potential amperometry (rCBF). Mild hypoxia and hyperoxia produced rapid changes (decrease and increase respectively) in the in vivo O₂ signal. Neuronal activation (tail pinch and stimulated grooming) produced similar increases in both O₂and rCBF indicating that CPE O₂currents provide an index of increases in rCBF when such increases exceed O₂ utilization. Saline injection produced a transient increase in the O₂ signal while chloral hydrate produced slower more long-lasting changes that accompanied the behavioral changes associated with anaesthesia. Acetazolamide increased O₂ levels through an increase in rCBF.

【 授权许可】

Unknown   
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