Surface Modification for Microreactor Fabrication

Sensors

Dorota G. Pijanowska²  El់ieta Remiszewska²  Cecilia Pederzolli¹  Lorenzo Lunelli¹  Michele Vendano¹  Roberto Canteri¹  Konrad Dudziński²  Jerzy Kruk² 

[1] Centro Per La Ricerca Scientifica E Tecnologia, ITCirst, Via Sommarive 18, Povo, 38050 Trento, Italy;Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences, ul. Ks. Trojdena 4, 02-109 Warsaw, Poland

关键词: Microreactors;    surface modification;    enzyme immobilisation;    lab-on-a-chip;   

DOI  :  10.3390/s6040370

来源: mdpi

【 摘 要 】

In this paper, methods of surface modification of different supports, i.e. glass and polymeric beads for enzyme immobilisation are described. The developed method of enzyme immobilisation is based on Schiff's base formation between the amino groups on the enzyme surface and the aldehyde groups on the chemically modified surface of the supports. The surface of silicon modified by APTS and GOPS with immobilised enzyme was characterised by atomic force microscopy (AFM), time-of-flight secondary ion mass spectroscopy (ToF-SIMS) and infrared spectroscopy (FTIR). The supports with immobilised enzyme (urease) were also tested in combination with microreactors fabricated in silicon and Perspex, operating in a flow-through system. For microreactors filled with urease immobilised on glass beads (Sigma) and on polymeric beads (PAN), a very high and stable signal (pH change) was obtained. The developed method of urease immobilisation can be stated to be very effective.

【 授权许可】

Unknown   
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