International Journal of Environmental Research and Public Health | |
Microbial Diversity and Bioremediation of a Hydrocarbon-Contaminated Aquifer (Vega Baja, Puerto Rico) | |
Enid M. Rodríguez-Martínez2  Ernie X. Pérez2  Christopher W. Schadt1  Jizhong Zhou1  | |
[1] Environmental Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37830, USA;Department of Biology, University of Puerto Rico, P.O. Box 9012, Mayagüez, PR 00681, Puerto Rico | |
关键词: Bioremediation; functional gene microarray; biofilm; tropical environments; | |
DOI : 10.3390/ijerph2006030036 | |
来源: mdpi | |
【 摘 要 】
Hydrocarbon contamination of groundwater resources has become a major environmental and human health concern in many parts of the world. Our objectives were to employ both culture and culture-independent techniques to characterize the dynamics of microbial community structure within a fluidized bed reactor used to bioremediate a diesel-contaminated groundwater in a tropical environment. Under normal operating conditions, 97 to 99% of total hydrocarbons were removed with only 14 min hydraulic retention time. Over 25 different cultures were isolated from the treatment unit (96% which utilized diesel constituents as sole carbon source). Approximately 20% of the isolates were also capable of complete denitrification to nitrogen gas. Sequence analysis of 16S rDNA demonstrated ample diversity with most belonging to the ∝, β and γ subdivision of the
【 授权许可】
CC BY
© 2006 MDPI. All rights reserved.
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Files | Size | Format | View |
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RO202003190059386ZK.pdf | 295KB | download |