Full Analytic Progress Curves of Enzymic Reactions in Vitro

International Journal of Molecular Sciences

Mihai V. Putz²  Ana-Maria Lacrămă¹ 

[1] Laboratory of Biochemistry, Chemistry Department, West University of Timişoara, Pestalozzi Street No.16, Timişoara, RO-300115, Romania E-mails:;Laboratory of Computational and Structural Physical Chemistry, Chemistry Department, West University of Timişoara, Pestalozzi Street No.16, Timişoara, RO-300115, Romania E-mails:

关键词: Michaelis-Menten mechanism;    W-Lambert function;    Logistic function;    multi-substrate kinetics;    and fully competitive inhibition;   

DOI  :  10.3390/i7110469

来源: mdpi

【 摘 要 】

Assuming the in vitro conditions for the enzyme-catalyzed reactions, the basic Michaelis-Menten description is modified in a logistic (mathematical) manner such that the inherent limitations that appear in the previous method are removed. Beside its generality, the reliability of the present approach is proved through applications on the competitive multi- and bi- substrate enzyme catalyses.

【 授权许可】

CC BY   
© 2006 by MDPI (http://www.mdpi.org).