Identification and molecular modeling of a family 5 endocellulase from Thermus caldophilus GK24, a cellulolytic strain of Thermus thermophilus

International Journal of Molecular Sciences

Dooil Kim¹  Bo Hyun Park¹  Bo-Won Jung¹  Mi-kyung Kim¹  Suk-In Hong¹ 

[1] ¹Systems Microbiology Research Center, KRIBB, Daejeon 305-806, Korea ²Department of Biomicrosystem Technology, Korea University, Seoul 136-701, Korea ³Department of Chemical and Biological Engineering, Korea University, Seoul 136-701, Korea

关键词: Endocellulase;    Thermus caldophilus GK24;    Glycosyl hydrolase family 5;    Homology modeling;    Molecular dynamics;    Congo-red staining;   

DOI  :  10.3390/i7120571

来源: mdpi

【 摘 要 】

The genome of T. caldophilus GK24 was recently sequenced and annotated as 14contigs, equivalent to 2.3 mega basepairs (Mbp) of DNA. In the current study, we identifieda unique 13.7 kbp DNA sequence, which included the endocellulase gene of T. caldophilusGK24, which did not appear to be present in the complete genomic sequence of the closelyrelated species T. thermophilus HB27 and HB8. Congo-red staining revealed a uniquephenotype of cellulose degradation by strain GK24 that was distinct from other closelyrelated Thermus strains. The results showed that strain GK24 is an aerobic, thermophilic,cellulolytic eubacterium which belongs to the group T. thermophilus. In order to understandthe mechanism of production of cellobiose in T. caldophilus GK24, a three-dimensionalmodel of the endocellulase, TcCel5A, was generated based on known crystal structures.Using this model, we carried out a flexible cellotetraose docking study.

【 授权许可】

CC BY   
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.