International Journal of Molecular Sciences | |
Efficient Gene Transfection into Mammalian Cells Mediated by Cross-linked Polyethylenimine | |
Wei Dong1  Shufeng Li1  Guanghui Jin1  Qiming Sun1  Dingyuan Ma1  | |
[1] The State Key Laboratory of Pharmaceutical Biotechnology, College of Life Science, Nanjing University, Nanjing 210093, Jiangsu, P. R. China, E-mail: | |
关键词: Polyethylenimine; Gene delivery; Degradable; Cytotoxicity; B16F10; Mouse muscle; | |
DOI : 10.3390/i8020081 | |
来源: mdpi | |
【 摘 要 】
25 kDa branched polyethylenimine (PEI) has successfully been used for in vitro and in vivo gene delivery approaches, but it is cytotoxic. Smaller PEIs are usually non-cytotoxic but less efficient. In order to enhance the gene delivery efficiency and minimize cytotoxicity of PEI, we explored to synthesize cross-linked PEIs with degradable bonds by reacting amines of small branched 2000 Da PEI with small diacrylate (1,4-butanediol diacrylate or ethyleneglycol dimethacrylate) for 2–6 hours. The efficiency of the cross-linked PEIs during
【 授权许可】
Unknown
© 2007 by MDPI
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