DNA Optical Detection Based on Porous Silicon Technology: from Biosensors to Biochips

Sensors

Luca De Stefano²  Paolo Arcari¹  Annalisa Lamberti¹  Carmen Sanges¹  Lucia Rotiroti²  Ilaria Rea² 

[1] Department of Biochemistry and Medical Biotechnologies, University of Naples “Federico II”, Via S. Pansini 5, 80131 Napoli, Italy.;Institute for Microelectronics and Microsystems – Unit of Naples – National Council of Research, Via P. Castellino 111, 80131 Napoli, Italy (E-mail:

关键词: Optical Biosensors;    Porous Silicon;    Biochip;   

DOI  :  10.3390/s7020214

来源: mdpi

【 摘 要 】

A photochemical functionalization process which passivates the porous silicon surface of optical biosensors has been optimized as a function of the thickness and the porosity of the devices. The surface modification has been characterized by contact angle measurements. Fluorescence measurements have been used to investigate the stability of the DNA single strands bound to the nanostructured material. A dose-response curve for an optical label-free biosensor in the 6-80 mM range has been realized.

【 授权许可】

Unknown   
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