A Fluorimetric Sensor for Detection of One Living Cell

Sensors

Jan Vitecek¹  Jitka Petrlova³  Vojtech Adam³  Ladislav Havel¹  Karl J. Kramer⁴  Petr Babula² 

[1]Department of Plant Biology, and Faculty of Agronomy, Mendel University of Agriculture and Forestry in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic

[2]Department of Natural Drugs, University of Veterinary and Pharmaceutical Sciences, Palackeho 1-3, CZ-612 42 Brno, Czech Republic

[3]Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University of Agriculture and Forestry in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic

[4]Grain Marketing and Production Research Center, Agricultural Research Service, US Department of Agriculture, Manhattan, KS 66502, USA

关键词: Esterase;    Enzymes;    Single-cell analysis;    Attomole detection;    Fluorimetry;    Fluorescence microscopy;    Confocal microscopy;    Native gel electrophoresis;    Fluorescein diacetate;    Tobacco;    BY-2 cells;    Protoplast;    Growth curve;    Viability;   

DOI  :  10.3390/s7030222

来源: mdpi

【 摘 要 】

Nowadays, studies of metabolic pathways and processes in living organisms cannot be easily done at the cellular level. That is why the development of a new analytical methods and approaches is needed, to allow detection of different biologically important species at very low concentrations levels and sample volumes, especially in individual cells. In the present work, we suggested a sensor to detect units of living cells by means determination of plant esterases (PE) based on fluorimetric detection of the products of the enzymatic hydrolysis of fluorescein diacetate in plant cell cultures (BY-2 tobacco cells and early somatic embryos of Norway spruce, clone 2/32). We standardized the sensor using a readily available esterase from pig liver. The detection limits were approximately 17 to 50 amol in 2 ml (8.5 to 25 femtomolar concentrations of esterases) of the enzyme contained in BY-2 tobacco cells and spruce early somatic embryos, respectively, after re-computation on the amounts of pig liver esterases. We assumed that the optimised sensor for the determination of PE in cell extracts accomplishes all requirements for a sensitive analysis which could be usable for single cell analysis. The detection limit was 1.5 in case of analysing BY-2 tobacco cells and 0.5 in early somatic embryos. Moreover, we were able to detect single protoplasts.

【 授权许可】

Unknown   
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