Preparation of Silver Nanoparticle and Its Application to the Determination of ct-DNA

Sensors

Chunhua Liu¹  Xiupei Yang¹  Hongyan Yuan²  Zaide Zhou¹ 

[1] College of Chemistry, Sichuan University, Chengdu 610064, P. R. China;College of Chemical Engineering, Sichuan University, Chengdu 610064, P. R. China

关键词: silver;    nanoparticle;    fluorescence;    calf thymus DNA (ct-DNA);   

DOI  :  10.3390/s7050708

来源: mdpi

【 摘 要 】

new method to prepare silver nanoparticles was reported in this paper, which based on aqueous-gaseous phase reaction of silver nitrate solution and ammonia gas. The proposed method is effective, rapid, and convenient. Transmission electron microscope (TEM) and X-ray diffraction (XRD) were used to characterize the structure of the particles, respectively. It was found that the average diameter of the particles was about 10 nm and the shape was spherical. And the strong fluorescence signal of the silver nanoparticles solution can be quenched after the adding of the calf thymus DNA (ct-DNA) solution. The quenched fluorescence intensity was linear with the concentration of ct-DNA in the range of 0.5 to 5.0 × 10⁴ ng mL^-1 with a detection limit of 0.3 ng mL^-1.

【 授权许可】

Unknown   
© 2007 by MDPI (http://www.mdpi.org).