A Centrifugation-based Method for Preparation of Gold Nanoparticles and its Application in Biodetection

International Journal of Molecular Sciences

Zhiqiang Liang²  Juan Zhang¹  Lihua Wang¹  Shiping Song¹  Chunhai Fan¹ 

[1] Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China; E-mail:;Department of Biochemistry and National Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, China; E-mail:

关键词: gold nanoparticles;    stability;    centrifugation;    DNA;    self-assembly;   

DOI  :  10.3390/i8060526

来源: mdpi

【 摘 要 】

Gold nanoparticles (AuNPs) have found widespread applications in life sciences. While synthesis of monodispersed AuNPs has been fairly convenient by using chemical reduction of chloroauric acid by sodium citrate, we found that AuNPs of high quality and high concentrations were not readily obtained via this method. As an example, we showed that monodispersed 13-nm AuNPs were readily synthesized at relatively low concentrations (e.g. 3.5 nM); in contrast, 13-nm AuNPs of 17 nM obtained by the direct reduction method were irregularly shaped and not well dispersed. In this work, we demonstrated that AuNPs of high concentration could be prepared by a two-step approach, i.e. chemical reduction at low concentrations and subsequent centrifugation. Compared to the direct reduction method, this new two-step method led to AuNPs with high salt resistance and high stability, which are essential for the preparation of DNA-AuNPs conjugates for DNA biodetection.

【 授权许可】

Unknown   
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