Antiradical Activity of Paulownia tomentosa (Scrophulariaceae) Extracts

Molecules

Karel Smejkal¹  Pavla Holubova¹  Ales Zima¹  Jan Muselik² 

[1] Natural Drugs Department, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, Palackého 1-3, CZ-612 42 Brno, Czech Republic; E-mails: Pavla Holubová:;Department of Pharmaceutics, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, Palackého 1-3, CZ-612 42 Brno, Czech Republic; E-mail: Jan Muselík:

关键词: Paulownia tomentosa;    antioxidant;    DPPH;    antiperoxynitrite;    phenolics;   

DOI  :  10.3390/12061210

来源: mdpi

【 摘 要 】

Paulownia tomentosa is a large indecidous tree planted mostly for its fast growing wood and decoratative purposes. The tree is also used in traditional Chinese medicine. As a part of our study of natural polyphenols, the fruits of Paulownia tomentosa were extracted by EtOH and than subjected to liquid/liquid extraction. Fractions were analysed by TLC and HPLC to determine presence of phenolic substantaces. We identified and quantified acteoside (1) and isoacteoside (2) in the EtOAc and n-BuOH extracts; mimulone (3) and diplacone (4) in the MeOH extract. To determine the antiradical activity of extracts we used the anti DPPH and peroxynitrite assays. The activity was expressed as Trolox C equivalents, IC₅₀ for DPPH scavenging and a time dependency course was established. The polyphenols content was determined; results were expressed as gallic acid equivalents. Using these methods we found the fractions of the n-BuOH, EtOAc and MeOH extracts that display antiradical activity, which could be exploited as potential pharmaceuticals.

【 授权许可】

Unknown   
© 2007 by MDPI (http://www.mdpi.org).