Purification and Preliminary Crystallographic Analysis of a New Lys49-PLA2 from B. Jararacussu

International Journal of Molecular Sciences

Marcelo L. dos Santos²  Fปio H. R. Fagundes¹  Bruno R. F. Teixeira²  Marcos H. Toyama³ 

[1] Instituto de Biologia, Universidade Estadual de Campinas, Campinas-SP, Brazil;Laboratório de Biologia Estrutural e Cristalografia, Instituto de Química, Universidade Estadual de Campinas, CP 6154, 13083–970, Campinas-SP, Brazil;Laboratório de Química de Macromoléculas, UNESP/CLP, São Vicente-SP, Brazil

关键词: Lys49-PLA2;    Bothrops jararacussu;    platelet aggregation;    phospholipase crystallographic analysis;   

DOI  :  10.3390/ijms9050736

来源: mdpi

【 摘 要 】

BjVIII is a new myotoxic Lys49-PLA2 isolated from Bothrops jararacussu venom that exhibits atypical effects on human platelet aggregation. To better understand the mode of action of BjVIII, crystallographic studies were initiated. Two crystal forms were obtained, both containing two molecules in the asymmetric unit (ASU). Synchrotron radiation diffraction data were collected to 2.0 Å resolution and 1.9 Å resolution for crystals belonging to the space group P2₁2₁2₁ (a = 48.4 Å, b = 65.3 Å, c = 84.3 Å) and space group P3₁21 (a = b = 55.7 Å, c = 127.9 Å), respectively. Refinement is currently in progress and the refined structures are expected to shed light on the unusual platelet aggregation activity observed for BjVIII.

【 授权许可】

CC BY   
© 2008 by MDPI